Commercial production of micropropagated banana is. Acclimatization of plants transferred to soil micropropagation successful plant transfer from culture to soil show high survival rate and cost involved in the process is low. Micropropagation is defined as the production of whole plants from cell cultures derived from explants, according to biotechnology. Shoot culture 9most widely used method for commercial micropropagation 9relatively high genetic stability in the plants produced shoot culture advantages 9reliable rates and consistency of shoot multiplication 93 8 fold multiplication rate per month 9preexisting meristems are least susceptible to genetic changes micropropagation stages stage 0. Kinetin, micro propagation, in vitro shoot initiation. Micropropagation of some malaysian banana and plantain. Asiapacific consortium on agricultural biotechnology. The technique has been applied to a substantial number of commercial vegetatively propagated plant species. Ndakidemi1 1school of life sciences, nelson mandela african institute of science and technology, arusha, tanzania. Under bangladesh conditions, tissue culture derived plantlets of banana.
The requirement of large numbers of quality propagules for plantation made the. Pdf production of bananas in uganda using tissue culture. The shoot tips were cultured on ms media supplemented with different concen trations of bap 0, 2, 4, 6 and 8 mgl with or without iaa at concentration of 0. Banana plants grow notably slower with the presence of weeds because partial of the water and nutrients are absorbed by the weeds. Banana micropropagation medium provides all the essential macroelements and microelements. The in vivo clonal propagation is often difficult, expensive and even unsuccessful. Studies on in vitro micropropagation in banana semantic scholar. Optimizing the effect of plant growth regulators on in vitro micro. The yellow sweet banana cultivars most commonly found in western greengrocers are the focus of this biology document.
However, the productivity and lifespan of banana and plantain fields have drastically reduced due to pest and disease pressure. None of the in sterile distilled water and were then kept in a laminar mega data exchange xml format 70. When the pup is taken the cut must be into the mother banana plant enough to obtain some roots. Sweet bananas in general, however, show enormous diversity in terms of plant stature and fruit size, and fruit colour extends from yellow and green to. Low cost micropropagation package for banana international. A 90% resource cost reduction in tissue culture of banana was achieved by replacing tissue culture grade sucrose and gelrite in the medium with locally available commercial sugar and a starchgelrite mixture and by using sun light instead of. The formulation is a nutrient blend of inorganic salts, amino acid and vitamins. Biotechnology applications for banana and plantain.
In india banana yield is being developed in atmosphere running from damp tropical to dry mellow subtropics through choice of fitting assortments like grandnaine 125. Multiplication of genetically identical copies of a cultivar by asexual reproduction is called clonal propagation. The major reasons are cost of production and knowhow. Large leaves are cut off of the pup leaving only the youngest leaves or no leaves at all. Part of the methods in molecular biology book series mimb, volume 877 banana musa spp. Using explants with apical domes, a total of 22 banana cultivars were successfully. Micropropagation stages, types, applications, advantages. Aaa is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation.
Virus disease has greatly reduced the productivity of vegetatively propagated banana 6. Box 18 chiuju, pingtung, taiwan 90403 roc this paper discusses the micropropagation of banana plantlets in taiwan. Application of temporary immersion system in banana micropropagation s. In the past 20 years, a total of 40 million diseasefree plantlets have been supplied for commercial planting in taiwan.
However, the requirement of cytokinin and auxins depends on the variety of banana and culture conditions cronauer and krikorian, 1984a. Paclobutrazol abstract in 1983 a commercial micropropagation system was established in taiwan to produce disease free planting material to control the spread of wilt race fusarium. The micro propagation of shoot tips in vitro is a widespread application of biotechnology in agriculture. Potassium nitrate and ammonium nitrate serve as sources of. Micropropagation stages, types, applications, advantages, limitations. Banana is fundamentally a tropical product, develops well in.
Prospects of banana micropropagation for quality planting. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Article full text enhanced pdf format, 324724 bytes article sharing repository deposits. They are cultivated in areas of about 9 millions hectares spread throughout more than 100 countries, with an annual production of around 89 million metric tons fao, 2000.
Shoot tips isolated from the rhizomes were found to be suitable material for plantlet production in vitro. Effect of different phytohormones on micropropagation of. Sterilized runner tips were cultured on hormonefree murashige and skoog ms medium with 3% sucrose, 1 mll. Tissue culture propagation or micropropagation in merge fdf file pdf the commercial production of. Alternatively, freshly initiated cultures can be kept in complete darkness for one week. A training video showing you how banana plantlets can be safely and efficiently multiplied onsite using macropropagation technology. The in vitro propagation techniques for producing banana using shoot tip cultures munguatosha ngomuo1, emerald mneney2, patrick a. Micropropagation using male floral meristems showed less or no somaclonal variation as compared. Micropropagation of banana using a liquid culture system. Summary of presentation study on micropropagation of. Five 5 or six 6 manual weedings should suffice after which the growth of weeds is rather impossible when banana plants reached. Bananasmusaspp, including plantains and cooking bananas, are among the most important horticultural crops in tropical and subtropical regions of the world. Use of tissue culture to alleviate the supply of quality. The areas ranges from micropropagation of horticultural crops, ornamental and forest trees etc.
The process is carried in a unit known as a macro propagator that can be made from locally available materials the seedlings can be selected to have uniform size and they tolerate post establishment stress better than tissue cultured plants. Micropropagation of banana using a liquid culture system chifumi nagai hawaii agriculture research center august 24, 2007 38th annual hawaii banana industry association conference. Micropropagation for production of quality banana planting material in asiapacific. Asiapacific consortium on agricultural biotechnology apcoab. Growth of banana in vitro shoots was thus compared with or without yellowcellophanefilm shading. Banana micropropagation medium has been developed for the in vitro propagation of musa species, family musaceae.
Micropropagation of some elite cassava cultivars cassava manihot esculenta crantz is the fifth most important food crop in the world conventionally propagated by stem cuttings highquality cassava cuttings for planting are often in short supply in cdi, cassava production has been hampered by the use of poorquality, disease. Generally, cytokinin helps in shoot proliferation and auxins helps in rooting of proliferated shoots. Tissue culture on banana suckers and rice callus micropropagation 16,997 views. Jarret rl, rodriguez w, fernandez r 1985 evaluation, tissue culture. Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature, and a defined nutrient medium. The role of topolins in micropropagation and somaclonal variation of banana cultivars williams and grand naine musa spp. Asexual reproduction through multiplication of vegetative parts is the only method for the in vivo propagation of certain plants, as they do not produce viable seeds e. Banana suckers selected are excised to obtain approximately 4 inches 10 cm of inner pseudostem tissue containing the banana meristem, as described in detail in fig. Clonal propagation has been successfully applied for the propagation of apple, potato, tuberous and several ornamental plants. Stimulation of banana in vitro shoot growth by yellow. Micropropagation of some malaysian banana and plantain musa sp. Collect at least two suckers from each plant source, one for micropropagation and the other for a nursery farm for future keiki needs. Phd thesis on micropropagation thesis on micropropagation database musicmarketing masters thesis phd thesis on plant tissue culture dissertation plants thesis.
Pdf lowcost alternatives for the micropropagation of. Pdf banana is one of the most important fruit of bihar. Transplanted plantlets shoot immediately irrigated with inorganic nutrient solution and maintained under high humidity for initial 1015 days. Tissue culture banana seedlings ready for transplanting. Tissue culture micropropagation technique has been developed during the past two decades are now considered well established. Wash the suckers thoroughly in tap water, roots and leaf sheaths are removed, and basal.
The main purpose is to produce healthy plants that are free of virus diseases, particularly banana bunchy top and banana mosaic. Thidiazuron in the improvement of banana micropropagation sinwan lee taiwan banana research institute, p. Micro propagation, shoot tips, fresh sucker, tissue culture, ms medium. In india only 7 percent area under the tissue culture plantlets and remaining 93percent propagated by sucker. Micropropagation is refers to rapid asexual in vitro multiplication of a desired plant of banana. Financing institution are also interested to finance of commercial production of banana tissue culture. Application of temporary immersion system in banana. The resulting plants were vigorous, uniform, and capable of fruiting within 1 year from micropropagation.
In vitro micropropagation of banana 647 as regards yield performance, tissue cultured plants have been reported to produce 39% higher yield than plants from sword suckers pradeep et al. The possibility of clonal propagation of banana through tissue culture was explored. It is a capitalintensive industry, and in some cases the unit cost per plant becomes unaffordable. Photoautotrophic micropropagation gabe, the difference lies in the scale up, you can turn this into a closed system as large as you want it to be. Lee taiwan banana research institute the banana micropropagation system was set up in 1983. An efficient micropropagation system for strawberry cv. It had highest rate of shoot initiation and multiplication. Plantain and banana are important staples and source of income for the smallholders that grow them in the humid forest and midaltitude agroecologies of subsaharan africa. Micropropagation technology is more expensive than the conventional methods of plant propagation, and requires several types of skills. Asiapacific consortium on agricultural biotechnology apcoab, new.
Explants were taken from young suckers of field grown plants of var. Our laboratory has found that depending on the combination of culture vessel and gelling agent more economic methods can be chosen for successfully micropropagating banana. Micropropagation is one of the plant tissue culture techniques which is commonly used in producing banana tissue cultures. Musa consist of about 40 species and is distributed through india, new guinea, australia and southeast asia simmonds, 1962. The in vitro propagation techniques for producing banana.
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